Journal: Frontiers in Microbiology

Article Title: Synergistic inhibition of Streptococcus mutans biofilms by fluoride and epigallocatechin gallate: insights from multi-omics analysis

doi: 10.3389/fmicb.2026.1766833

Figure Lengend Snippet: Effects of NaF and EGCG on S. mutans biofilm. (A) Percentage of S. mutans biofilms inhibition when treated with NaF. (B) Percentage of S. mutans biofilms inhibition when treated with EGCG. (C) Illustration of checkerboard microdilution assays on S. mutans biofilms. HC, high concentrations, 62.5 ppm of fluoride, 0.5 mg/mL of EGCG. LC, low concentrations, 15.6 ppm of fluoride, 0.125 mg/mL of EGCG. (D) Percentage of S. mutans biofilms inhibition combining with NaF and EGCG.

Article Snippet: S. mutans UA159 (ATCC 700610) was routinely cultivated in brain heart infusion (BHI; Difco, USA) at 37 °C under anaerobic conditions (85% N2, 10% H2, 5% CO2).

Techniques: Inhibition

Journal: Microorganisms

Article Title: Chemical Characterization and Effect of a Lactobacilli-Postbiotic on Streptococcus mutans Biofilm In Vitro

doi: 10.3390/microorganisms12050843

Figure Lengend Snippet: Effect of L. rhamnosus LR-32 postbiotic on the growth of S. mutans determined by optical density (OD at 600 nm); * p < 0.05.

Article Snippet: Aliquots (250 μL) of L. rhamnosus LR-32 conditioned medium (prepared as described in item 2.1) or PBS and 250 μL of 10 8 CFU/mL of S. mutans UA130 (ATCC 700611) were inoculated into 5 mL of BHI broth with 0.12% glucose.

Techniques:

Journal: Microorganisms

Article Title: Chemical Characterization and Effect of a Lactobacilli-Postbiotic on Streptococcus mutans Biofilm In Vitro

doi: 10.3390/microorganisms12050843

Figure Lengend Snippet: ( A ) Count of viable bacteria in S. mutans biofilms (CFU/mL) formed with and without the postbiotic L. rhamnosus LR-32. ( B ) Dry weight of S. mutans biofilms formed (mg) with and without the postbiotic L. rhamnosus LR-32. The vertical bars indicate the standard deviation; * p < 0.05.

Article Snippet: Aliquots (250 μL) of L. rhamnosus LR-32 conditioned medium (prepared as described in item 2.1) or PBS and 250 μL of 10 8 CFU/mL of S. mutans UA130 (ATCC 700611) were inoculated into 5 mL of BHI broth with 0.12% glucose.

Techniques: Bacteria, Standard Deviation

Journal: Microorganisms

Article Title: Chemical Characterization and Effect of a Lactobacilli-Postbiotic on Streptococcus mutans Biofilm In Vitro

doi: 10.3390/microorganisms12050843

Figure Lengend Snippet: Relative gene expression (mean ± SD of foldchange) of the gtfB, gtfC, gtfD, and VicR genes of S. mutans with and without treatment with the postbiotic of L. rhamnosus LR-32 by RT-PCR; * p < 0.05.

Article Snippet: Aliquots (250 μL) of L. rhamnosus LR-32 conditioned medium (prepared as described in item 2.1) or PBS and 250 μL of 10 8 CFU/mL of S. mutans UA130 (ATCC 700611) were inoculated into 5 mL of BHI broth with 0.12% glucose.

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction